Dianthus NT.23

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Dianthus removes the complexity of binding interaction measurements for drug discovery screening. Get the fastest time to meaningful results with no immobilization required, because you demand it.

Dianthus NT.23 brings a breakthrough in novel biophysical tactic to high throughput screening approach and removes the complexity of binding interaction measurements for drug discovery, while providing a standardization and automation friendly 384-wellls plate format.

  • Find hits for any target type in any buffer or bioliquid
  • Consume the smallest amount of your target and library compounds
  • Measure the tightest interactions down to picomolar Kds

Dianthus NT.23 allows for:

  • Working with even the most challenging targets, previously inaccessible, regardless whether they are nucleic acids, multimeric proteins without enzymatic function or any other
  • Measuring affinities with the highest sensitivity spanning from pico- to millimolar range, covering all needs in the most projects
  • Ensuring minimal sample consumption, saving on expensive targets as well as allowing for very small test compounds use
  • Free use of literally any buffer of choice, even cell lysates or any other bioliquid, enabling the best possible scenario for studied drug discovery target corresponding to its most native state
  • Work in free solution, where no immobilization required, studied molecules are available for interactions in all dimensions and no extra time to work on expensive immobilizing chemistries is necessary
  • Test more than 8.000 compounds in duplicate in 24 hours and collect vast amount of meaningful data for further studies

Detected molecule range:

  • Molecular weight: 101-107 Daltons
  • Size: 0.1 nm – 1 μm

Minimum sample volume: 20 μL

Affinity range:

  • for Dianthus NT.23: nM to mM
  • for Dianthus NT.23Pico: pM to mM
  • for Dianthus NT.23PicoDuo: pM to mM

Run time for 384 data points:

  • for Dianthus NT.23: 60 min
  • for Dianthus NT.23Pico: 60 min
  • for Dianthus NT.23PicoDuo: 30 min

Format: 384-well plate (barcoded and sealable)

Fluorescence channels:

  • for Dianthus NT.23: 1 (Red)
  • for Dianthus NT.23Pico: 1 (Pico Red)
  • for Dianthus NT.23PicoDuo: 2 (Pico Red)

Temperature control: 20-25°C

Dimensions: 60cm W x 40cm H x 55cm D

Automation: Option to integrate with liquid handlers and robotics

Walk-away time (using 50-plate feeder):

  • for Dianthus NT.23: 50 hrs
  • for Dianthus NT.23Pico: 50 hrs
  • for Dianthus NT.23PicoDuo: 25 hrs

Compounds tested in 24 h single-dose in duplicate:

  • for Dianthus NT.23: ~ 4150
  • for Dianthus NT.23Pico: ~ 4150
  • for Dianthus NT.23PicoDuo: ~ 8300

Kds measured in 24 h 12-pt dilution series:

  • for Dianthus NT.23: ~ 750
  • for Dianthus NT.23Pico: ~ 750
  • for Dianthus NT.23PicoDuo: ~ 1500

TRIC stands for Temperature-Related Intensity-Change and describes how the fluorescence intensity of a fluorophore depends on the local temperature of the solution. For the vast majority of fluorophores, the fluorescence intensity decreases with increasing temperature. Most importantly, however, the extent of the temperature dependence is strongly related to the chemical environment of the fluorophore. This environment is sensitively affected by the binding of a ligand molecule to the fluorescently labeled target molecule.

MicroScale Thermophoresis, Thermophoresis, Affinity, Molecular Interaction, Binding Strength, MST