ImageXpress Pico

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Cellular imaging and analysis system that enables you to go from sample to results in minutes



Digital microscopy with automated brightfield, fluorescence, and Digital Confocal imaging

The ImageXpress Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so you can start running experiments quickly. With features such as Digital Confocal 2D on-the-fly deconvolution, Live Preview, and multi-wavelength cell scoring, the ImageXpress Pico offers you the ability to advance your discoveries in a small, affordable imager.

Get started quickly

With the icon-driven, user-friendly CellReporterXpress Image Acquisition and Analysis Software, your entire lab can streamline their digital microscopy. Start capturing and analyzing images with minimal training.

Do more than cell counting

Expand your assays with over 25 preconfigured templates optimized for many cell-based experiments including apoptosis, mitochondrial evaluation, 3D cell models, live cell/timelapse, multiwavelength cell scoring, and neurite tracing.

Automate imaging affordably

Alleviate the hassle of going to the core lab to run your samples. The system’s lab-friendly price allows researchers to afford the convenience of automated imaging and analysis on their lab bench. With options like Digital Confocal, environmental control and z-stack acquisition, the system can be ordered to fit your research.

Imaging modes: Transmitted light (brightfield), colorimetric, fluorescence, live-preview

Illumination: High power LEDs with >20,000 hour life

Objectives: 6 position automated turret with user-exchangeable objectives. Optics by Leica Microsystems: FLUOTAR 4x/NA
0.13, 10x/NA 0.32, 20x/NA 0.40, 40x/NA 0.60, 63x/NA 0.70

Camera: Sony CMOS, 5 megapixel

Channels: Cy5, TRITC, FITC, DAPI, Texas Red, CFP, white light, and RGB

Stage resolution: 0.625 μm

Imaging method: Single color, multicolor, time lapse, z-stacking, and Digital Confocal* 2D on-the-fly deconvolution

Autofocus method: LED autofocus or image autofocus with LED assist

Supported labware: 6- to 384-well plates and 25 mm x 75 mm (1 in. x 3 in.) slides, 35 mm culture dishes

Supported operating systems: Windows 10 (main computer), Windows 10 and macOS (clients)

Image output: 16-bit TIFF, JPG, MP4

Dimensions (in): 17.8 (H) x 21.7 (W) x 17.1 (D)

Dimensions (cm): 45.3 (H) x 55.1 (W) x 43.5 (D)

Weight (kg): 38 kg (84 lb) including options

Power: 100 VAC to 240 VAC, 50/60 Hz, 1.6A nominal at 115V, 200 Watts Max

Ambient operating temperature: 18°C to 30°C (59°F to 86°F)

Temperature control: Ambient +8°C to 40°C

Temperature control homogeneity: 37°C ± 0.5°C at 23°C ambient

Gas control: O2 control, 1-15% and ambient, CO2 control, ambient to 15%

Humidity control: Active humidity control. Sample compartment controlled to 85% relative humidity.

Multiple imaging modes

The ImageXpress Pico system offers objectives ranging from 4x to 63x and can operate in colorimetric, brightfield, fluorescence, or Digital Confocal 2D on-the-fly deconvolution imaging modes.

Plate-to-individual cell view

Data can be visualized at multiple levels from plate overview to individual cells. A wide variety of data visualization tools from the plate and cellular level empower users to learn as much as possible from their images and assays.

Quickly and easily identify regions of interest

Live Preview simplifies identification of regions of interest, letting users pan around the sample and interactively adjust focus with a virtual joystick, saving time and effort.

Preconfigured analysis protocols

Over 25 preconfigured analysis protocols ranging from simple cell counting to sophisticated neurite tracing analysis. With features like the click-to-find tool, analysis parameters can be optimized by simply clicking on a few cells that match specific criteria.

Z-stack acquisition

Generate sharper images for more accurate segmentation using z-stack acquisition. Acquire a series of images at different focal points to capture more detail than with a single slice. Users can include all slices or select which slices to include in the final projection.

Environmental control

Multi-day, time lapse, and live cell assays can be run using the onboard environmental system with options for humidity, CO2, and Ocontrol. Optimized to prevent z-drift, the software also provides real-time monitoring of environmental state, ensuring optimal assay conditions.