Glacios

Sample Screening and Data Acquisition for Single Particle Analysis Made Easy


The new Thermo Scientific™ Glacios™ Cryo Transmission Electron Microscope (Cryo-TEM) delivers a complete and affordable Cryo-EM solution to a broad range of scientists. It features 200 kV XFEG optics, the industry-leading Autoloader (cryogenic sample manipulation robot) and the same innovative automation for ease of use as on the Krios G3i Cryo-TEM. The Glacios Cryo-TEM bundles all this into a small footprint that simplifies installation.
The designed-in connectivity ensures a robust and contamination-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing. The Glacios Cryo-TEM can used in a single particle analysis (SPA) workflow for pre-screening of sample quality before transferring to the Krios Cryo-TEM for ultimate-resolution SPA data acquisition. Furthermore the Glacios Cryo-TEM can also be configured with a Direct Electron Detector and/or Volta Phase Plate to be a complete standalone SPA data acquisition solution.


Small footprint
The new hardware architecture of the Glacios has been specifically designed to address the need for a smaller footprint and easier access path. In many cases, this design avoids the additional investment and unwanted lab downtime for significant modifications to an existing infrastructure or the need for a purposely-built lab.

Enhanced ease of use through automation
The Glacios Cryo-TEM reduces the number of complex alignments that a user has to perform. For recurring alignments that need to be done regularly, automated alignment routines have been implemented into the EPU software for SPA. Furthermore the EPU user interface has been further streamlined to provide comprehensive user guidance.

Reproducible, optimal tool performance always guarantee
The optimal thermal and mechanical stability of the Glacios Cryo-TEM ensure perfect optical performance. The instrument features a self-assessment function that automatically evaluates the optical status of the microscope, providing feedback for any steps that require optimization. Additionally, automated alignment routines allow the instrument to be tuned to its optimal starting point for SPA or tomography experiments.

Maximum Throughput
EPU is the native software package for automated screening and acquisition of large data sets on the Thermo Fisher cryo-TEMs. With full control of the Autoloader from within EPU, all grids in a cassette can be batch-screened: after the creation of a grid atlas, ice quality (presence, thickness) of the vitrified grids is automatically categorized to support guided selection of grid squares.

  • Яркий катод X-FEG
  • Диапазон ускоряющих напряжений 80-200 кВ
  • Автозагрузчик на 12 сеточек
  • ПО для управления температурой, включая автоматическое пополнение дьюара и планирование охлаждения после крио-цикла
  • Автоматизированный конденсор, объектив и апертуры
  • Компьютеризированный 4-осевой столик с углом наклона +/- 70 градусов
  • Крио-столик с одноосевым держателем
  • Симметричная линза с постоянным напряжением для минимизации аббераций в процессе изменения режимов съемки и дифракции
  • Зазор (pole piece gap) 11 мм
  • Съемка без вращения в процессе изменения увеличения
  • Автоматическое отслеживание состояния оптических элементов микроскопа в сочетании с автоматической настройкой для оптимизации условий съемки
  • ПО EPU для SPA
  • Блок управления прибором, включающий два 24-дюймовых монитора, расположенный в соседнем помещении (не более 10 метров)
  • Digital FluCam: все ручные и автоматические настройки можно производить под контролем специальной камеры
  • Ceta 16М CMOS камера с динамическим диапазоном 16 бит
  • Операционная система Windows 7
  • Программный пакет Low dose для минимизации облучения электронами в процессе съемки
  • Защитный кожух.