Chirascan Q100

Applied Photophysics

Chirascan Q100 provides detailed insight into the Higher Order Structure (HOS) characteristics of complex biomolecules.


Reproducible robotics and high performance circular dichroism spectroscopy combine to generate quality data compatible with the most stringent statistical analysis methods. The result: objective, statistically-validated HOS comparisons.
Fully integrated for unattended operation, Chirascan Q100 saves days of operator time, analyzing 48 buffer-sample pairs over 24 hours whereas an experienced operator processes up to 14 samples per day on a manual system.


FULLY INTEGRATED, UNATTENDED OPERATION

  • Gain insight into Higher Order Structure (HOS) characteristics
  • Make objective, statistically-validated HOS comparisons
  • Achieve highest sensitivity and accuracy
  • Ready to run – save days of operator time
  • Obtain orthogonal data with simultaneous fluorescence measurements
  • Optimize sample concentration and absorbance

Performance characteristics
Spectral information: CD, absorbance as standard, Fluorescence and other detection modes available

Isothermal analysis, typical measuring time: Full spectrum < 2 min

Isothermal analysis, typical sample consumption:

  • Secondary structure (far-UV), 0.5 mm pathlength, cell width 9 mm: mAb 0.06 mg
  • Tertiary structure (near-UV), 10 mm pathlength, cell width 10 mm: mAb 2.8 mg
  • Tertiary structure (near-UV), 10 mm pathlength, cell width 4 mm: mAb 0.5 mg

Thermal denaturation (thermal ramping): Full spectrum per 1°C, continuous ramp rate 1°C/min

Light source: 150W air-cooled Xenon arc lamp

Monochromator: Two polarizing prisms to maximize light throughput

Detection: Avalanche photodiode

Wavelength range (Note: using quartz prisms within monochromator limit measurements to wavelengths > 163 nm):163 nm to 1150 nm (Typical wavelength range for biomolecule analysis 180 nm to 350 nm)

Wavelength resolution: ±0.1 nm

CD calibration:

  • Optics-based, multiwavelength
  • Accuracy ±1% determined across wavelength range (selected wavelengths)

Measurement error on absolute absorbance: < 0.01 AU (simultaneous measurement of CD and absorbance signals)

Bandwidth:

  • 160 nm: up to 2 nm
  • 180 nm: up to 4 nm
  • 200 nm: up to 7.5 nm
  • 240 nm: up to 16 nm

Bandwidth precision: ±0.1 nm at 267 nm

Stray light: < 3 ppm at 200 nm

Typical Root Mean Square (RMS) noise values, no sample in place, 1 nm bandwidth, 2 s digital integration time – no smoothing, no rolling average:

  • 0.03 mdeg at 185 nm
  • 0.03 mdeg at 250 nm
  • 0.03 mdeg at 500 nm

Baseline stability (16 h drift test): < 0.4 mdeg

Sample temperature during analysis, coolant at 15°C or above:

  • Hardware tolerance: -20°C to +105°C
  • Typical range for biomolecule analysis: 4°C to 95°C

Sample temperature during on-instrument storage: 0-70°C, accuracy +0.2°C

Data handling and storage

PC operating system: Microsoft® Windows® 7 Professional, 64 bit

Data storage and export: Storage in proprietary format, exportable as .csv

Compliance

Physical and environmental specifications

Instrument weight and dimensions (WxDxH): 160 kg, 195 x 65 x 120 cm

Operating conditions:

  • temperature 20 to 25°C controlled to within 1.5°C
  • humidity 20 to 80 % non-condensing

Nitrogen requirement (flow rate, pressure, purity): 5 L per min, > 4 bar, > 99.998%

Chirascan systems are delivered ready to run, including flow cells and/or cuvettes selected for the most common analytical conditions.