CellReporter
CellReporter facilitates
rapid, early decision making in compound and biotherapeutic screening
through its imaging speed, immediate data analysis and optimized cell
based assays.
You make screening decisions earlier and focus on leads, saving precious resource, time and money.
Benefits of CellReporter:
• Eliminate aberrant compounds earlier in the screening process
Add the fastest CCD imager/analyzer to your workflow so that you make decisions earlier and focus only on leads.
Rapid, on-the-fly, high quality imaging of an entire 384-well plate in
3 channels, including autofocus, takes less than 10 minutes.
• Rapid imaging and analysis with immediate results presentation
Why wait for results? CellReporter instantly presents results in an easy-to-interpret format.
Through its imaging speed and immediate results presentation, CellReporter facilitates earlier decision-making saving precious resource, time and money.
• Amenable to all lab staff
To increase throughput, the system has been designed to be easy-to-use by anyone in the lab – no dedicated operators required.
Simple assay set-up, intuitive software and easy-to-interpret data presentation make the CellReporter system amenable to all lab staff.
• Optimized assays reduce cost
In synergy with the system, the optimized Astral Assays are tailored to the high sensitivity of CellReporter resulting in lower assay cost per well for screening.
• Flexible software to fit your workflow
CellReporter runs in two modes: Screen Manager and Image Acquirer. These are complementary and facilitate rapid results presentation.
Screen Manager
Screen Manager handles every step from plate layout templates through to interpretation of assay results (images, numbers and graphs).
Examples of applications that can be run within Screen Manager include:
• Astral Viability Assay;
• Astral Cytotoxicity Assay;
• Astral Cytotoxicity-Mono Assay (a single dye assay to determine the number of dead cells);
• Astral Apoptosis Assay (uses caspase activity to determine the extent of apoptosis in cell populations);
All-cell nuclear dye Apoptotic cells
• Proliferation Assay (detects proliferating cells by measuring new DNA synthesis in individual cells).
All cells Proliferating cells
Image Acquirer
Add the fastest CCD imager on the market to your workflow for the times when only rapid imaging is required. Use Image Acquirer to export cellular images from brightfield and up to 6 fluorescent wavelengths and analyze these in existing software packages and/or algorithms.
Applications that benefit from CellReporter include:
• Performing a range of cell-based assays;
• Optimizing or developing a new cell-based assay;
• Validating cell lines;
• Assessing transfection efficiency;
• Immunofluoresence e.g. below optimal conditions for immunofluorescent detection of a cytoplasmic protein.
• Broad white-light.
Image Acquisition:
• 4X, 10X and 20X objective lenses (automated rotation);
• Brightfield and 6 fluorescent filters (automated rotation);
• CCD detector;
• Entire 384-well plate in < 10 minutes, including autofocus, and 2 fluorescent channels.
Field of View:
• 4mm2 for 2μm resolution;
• 0.64mm2 for 0.8μm resolution;
• 0.16mm2 for 0.4μm resolution.
Software:
• Amenable to all lab staff;
• Simultaneous image acquisition and analysis delivers quantitative measurements of cell viability using the Astral Assays;
• Microscope-quality imaging combined with normalization against cell number and controls provides instant confidence in data generated;
• Manage your compound screening from plate templates through to compound ranking;
• IC50 curve generation and instant access to respective well images.
Cell Culture Range:
• Handles SBS standard for 96- and 384-well formats.
Astral Assays:
• Optimized assays to easily and affordably assess viability and cytotoxicity.
Genetix range of CellReporter assays have been produced to facilitate the screening of compounds for adverse effects on cultured cells.
Astral Cell Based Assay Kits
The Astral Assays constitute a suite of assays optimized to rapidly determine detrimental effects of compounds/biotherapeutics on cell proliferation. The Astral Assays have been optimized to the high sensitivity of CellReporter, thereby reducing the assay cost per well.
PetriWell cell culture plates
A family of optically enhanced cell culture plates specifically designed for automation.
CellReporter Related Systems:
CloneSelect Imager:
CloneSelect Imager revolutionizes cell line management; white light, label free, measuring confluence and cell growth objectively and consistently, performing rapid, detailed analysis of cells.
A bench top system, CloneSelect Imager measures confluence and cell growth, utilizing proprietary white-light imaging techniques to enable the non-invasive confluence determination of both adherent cells in microplate formats and suspended cells in static culture.
